Transcript abundance in two-row malting barley on interaction with fusarium graminearum chemotypes.
Crop Types
Collaborating Locations
University of ManitobaBackground
Fusarium head blight (FHB) is currently the most important disease in barley. Barley production has suffered millions of dollars in economic losses each year due to FHB. Fusarium not only damages the kernels, reducing crop yield and quality, but also produces mycotoxins that can render the grain unacceptable for malting and brewing, swine feed, or human consumption. Identifying genetic resistance in barley to FHB has been a challenge due to the quantitative nature of resistance and there has been no germplasm found which provides immunity to FHB. The goal of this study was evaluate new CDC barley germplasm and advanced CDC breeding lines for FHB resistance/low DON accumulation and to further develop the Near Infrared Reflectance Spectroscopy (NIR) calibration to predict grain DON content as a result of FHB.
Objectives
- Use RNA sequencing to see the different genetic responses of 2 barley cultivars when stressed by FHB infections.
Key Findings
Several gene targets were identified in ‘Norman’ that would be useful for breeding varieties with reduced deoxynivalenol DON content.
Key Takeaways
- This study generated ~ 360 GB of data in the form of sequences of the barley population. This data will allow for analysis of differential expression leading to a better understanding of FHB resistance in barley.